HNF4A antibody (N-Term)

Catalog No. ABIN2465821

This Goat Polyclonal antibody specifically detects HNF4A in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for HNF4A antibody (N-Term) (ABIN2465821)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HNF4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HNF4A Antibody

Binding Specificity: N-Term

Specificity: This antibody is expected to recognize the reported isoforms a, b and c (NP_849180.1, NP_000448.3, NP_849181.1 resp.).

Purification: HNF4A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: HNF4A antibody was raised against a 14 amino acid synthetic peptide near the N-Terminus of HNF4A.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 52 kDa and 48 kDa bands observed in lysates of human hepatocellular carcinoma cell line HepG2 (calculated MW of 52.8 kDa according to NP_000448.3 and 46.6 kDa according to NP_849181.1). Recommended concentration: 0.1-0.3 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4A

Gene ID: 3172

NCBI Accession: NP_849180, NP_000448, NP_849181

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process