Nestin antibody (AA 1484-1500)

Catalog No. ABIN2470324

This Rabbit Polyclonal antibody specifically detects Nestin in IHC, WB, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for Nestin antibody (AA 1484-1500) (ABIN2470324)

Target: Nestin (NES)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-Nestin Antibody

Binding Specificity: AA 1484-1500

Purification: Immunoaffinity Chromatography

Immunogen: Nestin antibody was raised against amino acids 1484 - 1500 of Nestin (Human).

Application Details

Application Notes: Nestin antibody can be used in ELISA starting at 1:1000 - 1:6000, Western Blot, and immunohistochemistry starting at 5 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies avoid freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store Nestin antibody at 4 °C or -20 °C.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

Background: Nestin, is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985). Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker, however, recent studies have found nestin expression in other cell types such as endothelial cells. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.

Gene ID: 10763

UniProt: P48681