Cholera Toxin beta antibody

Catalog No. ABIN2472974

Product Details anti-Cholera Toxin beta Antibody

Target: Cholera Toxin beta

Reactivity: Vibrio cholerae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cholera Toxin beta antibody is un-conjugated

Application: ELISA, Immunodiffusion (ID)

Purpose: Rabbit anti cholera toxin beta antibody recognizes the beta subunit of cholera toxin.

Characteristics: Purified Ig

Purification: Purified

Immunogen: Purified choleragenoid

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 4.0 mg/mL

Buffer: Phosphate buffered saline, 0.1% Sodium Azide (NaN3)

Handling Advice: This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended.

Expiry Date: 18 months

References for anti-Cholera Toxin beta antibody (ABIN2472974)

Thangawng, Kim, Golden, Anderson, Robertson, Low, Ligler: "A hard microflow cytometer using groove-generated sheath flow for multiplexed bead and cell assays." in: Analytical and bioanalytical chemistry, Vol. 398, Issue 5, pp. 1871-81, (2010) (PubMed).

Target Details for Cholera Toxin beta

Target: Cholera Toxin beta

Abstract: Cholera Toxin beta Products

Background: The beta subunit of cholera toxin binds to a GM1-ganglioside receptor which is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin alpha subunit into cells. The holotoxin consists of a pentameric ring of beta subunits whose central pore is occupied by the alpha subunit. The alpha subunit contains two chains, A1 and A2, linked by a disulfide bridge. The alpha subunit (and cholera toxin) activates the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.

UniProt: P01556