V5 Epitope Tag antibody

Catalog No. ABIN2477047

The Mouse Monoclonal anti-V5 Epitope Tag antibody has been validated for WB, ELISA, IP, IF, IHC (fro), RIA and FACS. It is suitable to detect V5 Epitope Tag in samples from . There are 2+ publications available.

Quick Overview for V5 Epitope Tag antibody (ABIN2477047)

Target: V5 Epitope Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This V5 Epitope Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA), Flow Cytometry (FACS)

Clone: SV5-Pk1

Product Details anti-V5 Epitope Tag Antibody

Purpose: MOUSE ANTI V5-TAG

Characteristics: Mouse anti V5-Tag, clone SV5-Pk1 recognizes the sequence, IPNPLLGLD, present on the P/V proteins of the paramyxovirus, SV5 (Dunn et al.1999). Clone SV5-Pk1 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have labeled with tags containing this sequence (Randall et al.1993 and Zhao et al. 2005).

Purification: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Immunogen: Paramyxovirus Simian-Virus 5 (SV5)

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline,0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

References for anti-V5 Epitope Tag antibody (ABIN2477047)

Hornemann, Richard, Rütti, Wei, von Eckardstein: "Cloning and initial characterization of a new subunit for mammalian serine-palmitoyltransferase." in: The Journal of biological chemistry, Vol. 281, Issue 49, pp. 37275-81, (2006) (PubMed).

Young, Chatziandreou, He, Goodbourn, Lamb, Randall: "Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells." in: Journal of virology, Vol. 75, Issue 7, pp. 3363-70, (2001) (PubMed).

Target Details for V5 Epitope Tag

Target: V5 Epitope Tag

Alternative Name: V5-TAG

Target Type: Tag

Background: Synonyms: PK-TAG

UniProt: P11207