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HCN2 antibody (AA 761-863) (PerCP)

HCN2 Reactivity: Rat WB, IHC, IP, IF, ICC, AA Host: Mouse Monoclonal S71 PerCP
Catalog No. ABIN2481390
  • Target See all HCN2 Antibodies
    HCN2 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 2 (HCN2))
    Binding Specificity
    • 10
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 761-863
    Reactivity
    • 24
    • 21
    • 10
    • 1
    Rat
    Host
    • 26
    • 12
    Mouse
    Clonality
    • 26
    • 12
    Monoclonal
    Conjugate
    • 20
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HCN2 antibody is conjugated to PerCP
    Application
    • 29
    • 19
    • 15
    • 13
    • 13
    • 12
    • 10
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Antibody Array (AA)
    Specificity
    Detects ~95 kDa. No cross-reactivity against HCN1.
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Protein G Purified
    Immunogen
    Fusion protein amino acids 761-863 (cytoplasmic C-terminus) of rat HCN2
    Clone
    S71
    Isotype
    IgG1
    Top Product
    Discover our top product HCN2 Primary Antibody
  • Application Notes
    • WB (1:1000)
    • IHC (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN2481390 was sufficient for detection of HCN2 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    HCN2 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 2 (HCN2))
    Alternative Name
    HCN2 (HCN2 Products)
    Synonyms
    HCN2 antibody, BCNG-2 antibody, BCNG2 antibody, HAC-1 antibody, HAC1 antibody, trls antibody, hyperpolarization activated cyclic nucleotide-gated potassium channel 2 antibody, hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 antibody, hyperpolarization-activated, cyclic nucleotide-gated K+ 2 antibody, LOC428335 antibody, HCN2 antibody, Hcn2 antibody
    Background
    Hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) is an integral membrane protein that helps establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). Ion channels are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain (3).
    Gene ID
    114244
    NCBI Accession
    NP_446136
    UniProt
    Q9JKA9
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