Ankyrin G antibody (C-Term) (PE)

Catalog No. ABIN2482473

This Mouse Monoclonal antibody specifically detects Ankyrin G in WB, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for Ankyrin G antibody (C-Term) (PE) (ABIN2482473)

Target: Ankyrin G (ANK3) (Ankyrin 3, Node of Ranvier (Ankyrin G) (ANK3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ankyrin G antibody is conjugated to PE

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S106-20

Product Details anti-Ankyrin G Antibody (PE)

Binding Specificity: C-Term

Specificity: Detects >~200 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein 1000 C-terminal amino acids of human Ankyrin G (also known as ANK-3 or ankyrin-3) encompassing all of Ankyrin G with the exception of Ankyrin repeats

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2482473 was sufficient for detection of Ankyrin-G in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Ankyrin G

Target: Ankyrin G (ANK3) (Ankyrin 3, Node of Ranvier (Ankyrin G) (ANK3))

Alternative Name: Ankyrin G

Background: Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton (1). Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane.

Gene ID: 288

NCBI Accession: NP_066267

UniProt: Q12955

Pathways: Synaptic Membrane, Maintenance of Protein Location, Skeletal Muscle Fiber Development