Ankyrin G antibody (AA 1690-1701)

Catalog No. ABIN462525

The Goat Polyclonal anti-Ankyrin G antibody (ABIN462525) specifically detects Ankyrin G in IHC and IHC (p). The antibody is reactive with Human, Monkey and Pig samples.

Quick Overview for Ankyrin G antibody (AA 1690-1701) (ABIN462525)

Target: Ankyrin G (ANK3) (Ankyrin 3, Node of Ranvier (Ankyrin G) (ANK3))

Reactivity: Human, Monkey, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Ankyrin G antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Ankyrin G Antibody

Binding Specificity: AA 1690-1701

Specificity: Human ANK3. This antibody is expected to recognise both reported isoforms (NP_001140.2, NP_066267.2)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Pig (100%) Orangutan, Gibbon, Elephant, Panda, Bovine, Dog, Horse (92%) Hamster, Bat, Rabbit (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-SWQNETSSGNLE from an internal region of human ANK3 (NP_066267.2, NP_001140.2). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Pig (100%), Orangutan, Gibbon, Elephant, Panda, Bovine, Dog, Horse (92%), Hamster, Bat, Rabbit (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Ankyrin G

Target: Ankyrin G (ANK3) (Ankyrin 3, Node of Ranvier (Ankyrin G) (ANK3))

Alternative Name: ANK3 / ANKYRIN-G

Background: Name/Gene ID: ANK3

Synonyms: ANK3, ANKYRIN-G, ANK-3, Ankyrin G119, Ankyrin-3

Gene ID: 288

NCBI Accession: NP_066267, NP_001140

UniProt: Q12955

Pathways: Synaptic Membrane, Maintenance of Protein Location, Skeletal Muscle Fiber Development