GRIN1/NMDAR1 antibody (AA 42-361) (Atto 488)

Catalog No. ABIN2483581

The Mouse Monoclonal anti-GRIN1/NMDAR1 antibody has been validated for WB, IHC, IF and ICC. It is suitable to detect GRIN1/NMDAR1 in samples from Rat.

Quick Overview for GRIN1/NMDAR1 antibody (AA 42-361) (Atto 488) (ABIN2483581)

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRIN1/NMDAR1 antibody is conjugated to Atto 488

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: S308-48

Product Details anti-GRIN1/NMDAR1 Antibody (Atto 488)

Binding Specificity: AA 42-361

Specificity: Detects ~105 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 42-361 (extracellular N-terminus) of rat NR1

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2483581 was sufficient for detection of NR1 glutamate receptor in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for GRIN1/NMDAR1

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Alternative Name: GluN1

Background: The NMDA receptor (NMDAR), a glutamate receptor, is the predominant molecular device for controlling synaptic plasticity and memory function (1). The NMDA receptor forms a heterotetramer between two NR1 and two NR2 subunits (the subunits are also called glutamate-binding NMDA receptor subunits or GluN for short), two obligatory NR1 subunits and two regionally localized NR2 subunits. A related gene family of NR3 A and B subunits have an inhibitory effect on receptor activity. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits.

Gene ID: 24408

NCBI Accession: NP_058706

UniProt: P35439

Pathways: Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity