ASIC1 antibody (AA 460-526) (PE)

Catalog No. ABIN2483697

This Mouse Monoclonal antibody specifically detects ASIC1 in WB, IHC, ICC and IF. It exhibits reactivity toward Mouse.

Quick Overview for ASIC1 antibody (AA 460-526) (PE) (ABIN2483697)

Target: ASIC1 (ACCN2) (Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ASIC1 antibody is conjugated to PE

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S271-44

Product Details anti-ASIC1 Antibody (PE)

Binding Specificity: AA 460-526

Specificity: Detects ~60 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 460-526 (Cytoplasmic C-terminus) of mouse ASIC1

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2483697 was sufficient for detection of ASIC1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for ASIC1

Target: ASIC1 (ACCN2) (Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))

Alternative Name: ASIC1

Background: Acid sensing ion channel ASIC1, also designated ACCN2, BNAC2 and ASIC1a, is present in brain as a 4.3-kb transcript with localization to rat dorsal root ganglia. Insitu hybridization of rat brain suggests that ASIC1 is most abundant in the main olfactory bulb, cerebral cortex, hippocampal formation, habenula, basolateral amygdaloid nuclei and cerebellum. ASIC1 and H+-gated currents may contribute to the development of fear and anxiety.

Gene ID: 11419

NCBI Accession: NP_033727

UniProt: Q6NXK8