ASIC1 antibody (N-Term)

Catalog No. ABIN2776229

Product Details anti-ASIC1 Antibody

Target: ASIC1 (ACCN2) (Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))

Binding Specificity: N-Term

Reactivity: Mouse, Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASIC1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MELKAEEEEV GGVQPVSIQA FASSSTLHGL AHIFSYERLS LKRALWALCF

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ACCN2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACCN2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 574 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ASIC1

Target: ASIC1 (ACCN2) (Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))

Alternative Name: ACCN2 (ACCN2 Products)

Synonyms: ACCN2 antibody, ASIC antibody, BNaC2 antibody, Accn2 antibody, ASIC1 antibody, AI843610 antibody, ASIC1a antibody, B530003N02Rik antibody, acid sensing ion channel subunit 1 antibody, acid-sensing (proton-gated) ion channel 1 antibody, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 antibody, ASIC1 antibody, Asic1 antibody, asic1 antibody, SMARCD1 antibody

Background: ACCN2 is the cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. ACCN2 is also permeable for Ca2+, Li+ and K+. ACCN2 generates a biphasic current with a fast inactivating and a slow sustained phase. ACCN2 mediates glutamate-independent Ca2+ entry into neurons upon acidosis. This Ca2+ overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. ACCN2 functions as a postsynaptic proton receptor that influences intracellular Ca2+ concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. It modulates activity in the circuits underlying innate fear.This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit, however, its function as an ion channel remains unknown. Alternative splicing of this gene generates 2 transcript products.
Alias Symbols: ASIC, ASIC1A, BNaC2, hBNaC2, ACCN2
Protein Interaction Partner: ATXN3, PICK1, ASIC1, ASIC2, PRKACA, STOM, STOML1,
Protein Size: 574

Molecular Weight: 65 kDa

Gene ID: 41

NCBI Accession: NM_020039, NP_064423

UniProt: P78348