KCC2 antibody (AA 932-1043) (Atto 594)

Catalog No. ABIN2484110

The Atto 594-conjugated Mouse Monoclonal anti-KCC2 antibody (Clone S1-12) (ABIN2484110) specifically detects KCC2 in IHC, WB, IP, ICC and IF. The antibody is reactive with Rat samples.

Quick Overview for KCC2 antibody (AA 932-1043) (Atto 594) (ABIN2484110)

Target: KCC2 (SLC12A5) (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 5 (SLC12A5))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCC2 antibody is conjugated to Atto 594

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S1-12

Product Details anti-KCC2 Antibody (Atto 594)

Binding Specificity: AA 932-1043

Specificity: Detects ~140 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 932-1043 corresponding to rat KCC2

Isotype: IgG2a

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:300)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2484110 was sufficient for detection of KCC2 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for KCC2

Target: KCC2 (SLC12A5) (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 5 (SLC12A5))

Alternative Name: KCC2

Background: KCC2 is a member of the cation-chloride cotransporter gene family (1). It acts as a K-Cl cotransporter. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain (2, 3). KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord (4). Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.

Gene ID: 171373

NCBI Accession: NP_599190

UniProt: Q63633