HMOX1 antibody (HRP)

Catalog No. ABIN2484289

The Rabbit Polyclonal anti-HMOX1 antibody has been validated for WB, IHC, ICC and IF. It is suitable to detect HMOX1 in samples from Human.

Quick Overview for HMOX1 antibody (HRP) (ABIN2484289)

Target: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMOX1 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-HMOX1 Antibody (HRP)

Specificity: Detects ~32 kDa.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: Human heme-oxygenase (HO-1) synthetic multiple antigenic peptide

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2484289 was sufficient for detection of HO-1 in 10 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for HMOX1

Target: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Alternative Name: HO-1

Background: Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator, biliverdin and bilirubin are potent antioxidants, and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3, however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).

Gene ID: 3162

NCBI Accession: NP_002124

UniProt: P09601

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome