PDIA3 antibody (Atto 594)

Catalog No. ABIN2484399

This Mouse Monoclonal antibody specifically detects PDIA3 in WB, IHC, IF, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for PDIA3 antibody (Atto 594) (ABIN2484399)

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDIA3 antibody is conjugated to Atto 594

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: Map-ERp57

Product Details anti-PDIA3 Antibody (Atto 594)

Specificity: Detects ~57 kDa.

Cross-Reactivity: Cow, Dog, Guinea Pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat

Purification: Protein G Purified

Immunogen: Human recombinant ERp57 (Grp58)

Isotype: IgG1

Application Details

Application Notes:

• WB (1:2000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

0.5 μg/ml of ABIN2484399 was sufficient for detection of ERp57 in 10 μg of heat shock Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for PDIA3

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Alternative Name: ERp57

Background: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).

Gene ID: 2923

NCBI Accession: NP_005304

UniProt: P30101

Pathways: Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis