ANK2 antibody (AA 203-496) (Biotin)

Catalog No. ABIN2485089

This Mouse Monoclonal antibody specifically detects ANK2 in IHC, WB, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for ANK2 antibody (AA 203-496) (Biotin) (ABIN2485089)

Target: ANK2 (Ankyrin 2, Neuronal (ANK2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ANK2 antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S105-13

Product Details anti-ANK2 Antibody (Biotin)

Binding Specificity: AA 203-496

Specificity: Detects ~200 kDa. No cross reactivity against Ankyrin-G.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Synthetic peptide amino acids 203-496 of human Ankyrin-B

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:200)
• ICC/IF (1:200)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2485089 was sufficient for detection of Ankyrin-B in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for ANK2

Target: ANK2 (Ankyrin 2, Neuronal (ANK2))

Alternative Name: Ankyrin B

Background: Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton (1). Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane.

Gene ID: 287

NCBI Accession: NP_001120965

UniProt: Q01484

Pathways: Cell-Cell Junction Organization