SPTBN4 antibody (AA 1621-1832)

Catalog No. ABIN2485663

The Mouse Monoclonal anti-SPTBN4 antibody is suitable to detect SPTBN4 in samples from Human. It has been validated for IHC, WB, ICC and IF.

Quick Overview for SPTBN4 antibody (AA 1621-1832) (ABIN2485663)

Target: SPTBN4 (Spectrin Beta, Non-Erythrocytic 4 (SPTBN4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPTBN4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S393-2

Product Details anti-SPTBN4 Antibody

Binding Specificity: AA 1621-1832

Specificity: Detects ~>200 kDa. Does not cross react with other Beta-spectrins

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:100 dilution of ABIN2485663 was sufficient for detection of Beta 4 Spectrin in 20 μg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for SPTBN4

Target: SPTBN4 (Spectrin Beta, Non-Erythrocytic 4 (SPTBN4))

Alternative Name: beta 4 Spectrin

Background: Spectrin is the major constituent of the cytoskeletal network underlying the erthrocyte plasma membrane and determines cell shape, arrangement of transmembrane proteins and organization of organelles. It is expressed at very low levels in many tissues, with the strongest expression in the cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder and heart (1). Spectrin beta 4 is a non-erythrocyte member, and is expressed in the brain and pancreatic islets and localisted to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Beta4 spectrins are also essential for membrane stability and the molecular organization of the nodes of Ranvier (2).

Gene ID: 57731

NCBI Accession: NP_066022

UniProt: Q9H254

Pathways: Sensory Perception of Sound, Regulation of Actin Filament Polymerization, Maintenance of Protein Location