Amyloid antibody (APC)

Catalog No. ABIN2486477

Product Details

Target: Amyloid

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: APC

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Recognizes all types of amyloid oligomers. Appears to recog a peptide backbone epitope common to amyloid oligomers, but not found in native proteins, amyloidogenic monomer or mature amyloid fibrils.

Cross-Reactivity: Eukaryotes, Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: Synthetic molecular mimic of soluble oligomers

Application Details

Application Notes:

• WB (1:200)
• IP (1:200)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:200 dilution of ABIN2486477 was sufficient for detection of amyloid oligomers in 10 μg of mouse brain lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details

Target: Amyloid

Synonyms: amyloid beta precursor protein antibody, App antibody

Background: Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibres (1, 2). These include the amyloid-β peptide plaques and tau neurofibrillary tangles in senile plaques of Alzheimer's symptomology, the deposition of α-synuclein in the Lewy bodies of Parkinson's disease, and accumulation of polyglutamine-containing aggregates in Huntington's disease (2, 3).

Gene ID: 8666

NCBI Accession: NM_000484

UniProt: P05067