GRP78 antibody (HRP)

Catalog No. ABIN2486760

This Rabbit Polyclonal antibody specifically detects GRP78 in WB, IHC, ICC, IF and IP. It exhibits reactivity toward Rat.

Quick Overview for GRP78 antibody (HRP) (ABIN2486760)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRP78 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-GRP78 Antibody (HRP)

Specificity: Detects ~78 kDa.

Cross-Reactivity: Cow, Dog, Fusarium, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Xenopus laevis

Purification: Peptide Affinity Purified

Immunogen: Rat GRP78 (Bip) synthetic peptide conjugated to KLH

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN2486760 was sufficient for detection of Grp78 in 10 μg of rat tissue lysate by ECL immunoblot analysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: GRP78

Background: GRP78 is a ubiquitously expressed, 78- kDa glucose regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).

Gene ID: 25617

NCBI Accession: NP_037215

UniProt: P06761

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling