Xylose antibody

Catalog No. ABIN249329

The Rabbit Polyclonal anti-Xylose antibody is suitable to detect Xylose in samples from Chlamydomonas and Higher Plants. It has been validated for WB.

Quick Overview for Xylose antibody (ABIN249329)

Target: Xylose

Reactivity: Chlamydomonas, Higher Plants

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details

Purpose: Anti-Xylose

Specificity: Antibody binds to heavy chains of Goat IgG and light chains of all Goat immunoglobuli

Cross-Reactivity (Details): Higher plants and algae
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Higher plants

Purification: Immunogen affinity purified serum

Immunogen: xylose residues bound to the N-glycan in beta 1

Application Details

Application Notes: 1 μg/mL (ELISA), 2 μg/10 mL incubation buffer (WB)

Comment:

negative control: Fetuin, a glycoprotein containing fucose linked in alpha 1.6 and no xylose, Sigma, product number F3385.positive control: Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: Xylose

Background:

Background: This antibody specifically cross-reacts against xylose residues bound to the protein N-glycans in beta1,2. This residue is characterisitc of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.

Additional information: Beta (1,2) xylose is present exclusively in plant N-glycans so antibodies against this sugar moiety should not cross-react with any mammal glycoprotein.This antibody do not bind free D-xylose. This antibody does not seem to work in immunolocalization.

Molecular Weight: 10-100 for various glycoproteins