Small Subunit X of Psii (Psbx) antibody

Catalog No. ABIN249330

The Rabbit Polyclonal anti-Small Subunit X of Psii (Psbx) antibody (ABIN249330) specifically detects Small Subunit X of Psii (Psbx) in WB. The antibody is reactive with Arabidopsis thaliana samples.

Quick Overview for Small Subunit X of Psii (Psbx) antibody (ABIN249330)

Target: Small Subunit X of Psii (Psbx)

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details

Purpose: Anti-PsbX | small subunit X of PSII

Cross-Reactivity (Details): Arabidopsis thaliana
Not reactive in: Cyanobacteria

Predicted Reactivity: Dictos,Oryza sativa,Zea mays

Purification: Serum

Immunogen: KLH-conjugated synthetic peptide dervied from Arabidopsis thaliana PsbX sequence UniProt: Q9SKI3 TAIR: At2g06520

Application Details

Application Notes: 1 : 2500, 1 μg of chlorophyll/lane (WB)

Comment:

For an image of antibody detection in a western blot application see Garcia-Cerdan et al. (2008).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: Small Subunit X of Psii (Psbx)

Background:

Background: Plant PsbX is a nucelar encoded small 4 kDa protein associated with Photosystem II and found in all classes of oxygenic organisms.

Additional information: PsbX is a small (4 kDa) and very hydrophobic subunit of PSII, Immunoblots from SDS-gels (especially with high loading) may show higher molecular weight signals from PsbX not fully detached from other subunits of PSII, The use of 15 % SDS-PAGE gels with 2-4 M urea is recommended when working with this antibody

Molecular Weight: 4 kDa

Gene ID: 815210

UniProt: Q9SKI3