14-3-3-Like Protein C antibody

Catalog No. ABIN249336

Cited in 1 publication. The Rabbit Polyclonal anti-14-3-3-Like Protein C antibody (ABIN249336) specifically detects 14-3-3-Like Protein C in ELISA and WB. The antibody is reactive with Barley samples.

Quick Overview for 14-3-3-Like Protein C antibody (ABIN249336)

Target: 14-3-3-Like Protein C

Reactivity: Barley

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB)

Product Details

Purpose: Anti-14,3,3C | 14,3,3-like protein C

Cross-Reactivity (Details): Hordeum vulgare
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Horderum vulgare

Purification: Serum

Immunogen: synthetic peptide specific to the barley C isoform

Application Details

Application Notes: 1 : 15 000 (ELISA), 1 : 2000 (WB)

Comment:

specific for barley 14.3.3C isoform, when tested against 14.3.3A, B and C isoforms

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Pertl, Pöckl, Blaschke, Obermeyer: "Osmoregulation in Lilium pollen grains occurs via modulation of the plasma membrane H+ ATPase activity by 14-3-3 proteins." in: Plant physiology, Vol. 154, Issue 4, pp. 1921-8, (2010) (PubMed).

Target Details

Target: 14-3-3-Like Protein C

Alternative Name: 14.3.3-like protein C (14.3.3C)

Background: 14.3.3's are 30KDa proteins involved in protein interactions with target proteins containing phosphorylated target sites. Functions of 14.3.3's include acting as adaptors or scaffolds, stimulating protein-protein interaction, altering target protein activity, causing conformational changes of target proteins, regulating subcellular localisation and also facilitating transport (for example nuclear import/export and transport in the endomembrane system). A variety of apparently unrelated biological activities, including a role in development and signal transduction have been ascribed to the 14.3.3 family. To date, five barley 14.3.3 homologues have been identified and characterised and named 14.3.3A through E.

Molecular Weight: 30 kDa