Fucose antibody
Reactivity: Higher Plants
IL, WB
Host: Rabbit
Polyclonal
unconjugated
1 image
(1 reference)-
- Target
- Fucose
- Reactivity
- Higher Plants
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunolocalization (IL), Western Blotting (WB)
- Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- Predicted Reactivity
- higher plants
- Purification
- serum
- Immunogen
- core fucose residues bound to the N-glycan in alpha 1,3
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- Application Notes
- 1 μg/10 mL of incubation buffer, with standard ECL (WB), 1: 40 (IL)
- Comment
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controls:PLA2 (phospholipase 2 from bee venom) which contains only α1.3 fucose, Sigma, product number P9279Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250The antibody does not recognize alpha 1,6-fucose.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 μL of sterile water.
- Buffer
- PBS pH 7.4
- Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
- Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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-
: "N-glycans of Phaeodactylum tricornutum diatom and functional characterization of its N-acetylglucosaminyltransferase I enzyme." in: The Journal of biological chemistry, Vol. 286, Issue 8, pp. 6152-64, (2011) (PubMed).
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: "N-glycans of Phaeodactylum tricornutum diatom and functional characterization of its N-acetylglucosaminyltransferase I enzyme." in: The Journal of biological chemistry, Vol. 286, Issue 8, pp. 6152-64, (2011) (PubMed).
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- Target
- Fucose
- Background
- This antibody specifically cross-reacts against fucose residues bound to the protein N-glycans in alpha 1,3. This residue is characterisitc of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.
- Molecular Weight
- 10 - 100 for various glycoproteins
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