Fucose antibody

Catalog No. ABIN249420

Cited in 1 publication. The Rabbit Polyclonal anti-Fucose antibody (ABIN249420) specifically detects Fucose in ELISA, IL and WB. The antibody is reactive with Diatoms and Higher Plants samples.

Quick Overview for Fucose antibody (ABIN249420)

Target: Fucose

Reactivity: Diatoms, Higher Plants

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunolocalization (IL), Western Blotting (WB)

Product Details

Purpose: Anti-Fucose

Cross-Reactivity (Details): Higher plants, Pheodactylum tricornutum (diatom)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Higher plants,Phaeodactylum tricornutum

Purification: Immunogen affinity purified serum

Immunogen: Core fucose residues bound to the N-glycan in alpha 1

Application Details

Application Notes: 0.5 μg/mL (ELISA), 1 : 40 (IL), 1 μg/10 mL (WB)

Comment:

controls:PLA2 (phospholipase 2 from bee venom) which contains only α1.3 fucose, Sigma, product number P9279Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250The antibody does not recognize alpha 1,6-fucose.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Baïet, Burel, Saint-Jean, Louvet, Menu-Bouaouiche, Kiefer-Meyer, Mathieu-Rivet, Lefebvre, Castel, Carlier, Cadoret, Lerouge, Bardor: "N-glycans of Phaeodactylum tricornutum diatom and functional characterization of its N-acetylglucosaminyltransferase I enzyme." in: The Journal of biological chemistry, Vol. 286, Issue 8, pp. 6152-64, (2011) (PubMed).

Target Details

Target: Fucose

Background:

Background: This antibody specifically cross-reacts against fucose residues bound to the protein N-glycans in alpha 1,3. This residue is characteristic of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.

Additional information: Alpha (1,3) fucose is present not only in plants but also in some invertebrates (such as nematodes, bees, etc,) , However, cross-reaction with glycoproteins from these organisms is weaker than the one observed in plants, This sugar residue does not exist in mammals, in their endogenous glycoproteins

Molecular Weight: 10 - 100 for various glycoproteins