CBX4 antibody (AA 401-500)

Catalog No. ABIN2559520

This anti-CBX4 antibody is a Rabbit Polyclonal antibody detecting CBX4 in WB, ELISA, IF (cc), IF (p), IHC (p), ICC and IHC (fro). Suitable for Human and Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for CBX4 antibody (AA 401-500) (ABIN2559520)

Target: CBX4 (Chromobox Homolog 4 (CBX4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBX4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CBX4 Antibody

Binding Specificity: AA 401-500

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CBX4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CBX4 antibody (ABIN2559520)

Han, Wang, Li, Dong, Zhang, Yan: "HIF-1α SUMOylation affects the stability and transcriptional activity of HIF-1α in human lens epithelial cells." in: Graefe's archive for clinical and experimental ophthalmology, Vol. 253, Issue 8, pp. 1279-90, (2015) (PubMed).

Target Details for CBX4

Target: CBX4 (Chromobox Homolog 4 (CBX4))

Alternative Name: CBX4

Background:

Synonyms: PC2, NBP16, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Polycomb 2 homolog, hPc2, CBX4

Background: E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Gene ID: 8535

UniProt: O00257