DTX3L antibody (AA 651-740) (HRP)

Catalog No. ABIN2559610

The HRP-conjugated Rabbit Polyclonal anti-DTX3L antibody (ABIN2559610) specifically detects DTX3L in ELISA, IHC (p) and IHC (fro). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for DTX3L antibody (AA 651-740) (HRP) (ABIN2559610)

Target: DTX3L (Deltex E3 ubiquitin ligase 3L (DTX3L))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DTX3L antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DTX3L Antibody (HRP)

Binding Specificity: AA 651-740

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DTX3L

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DTX3L

Target: DTX3L (Deltex E3 ubiquitin ligase 3L (DTX3L))

Alternative Name: DTX3L

Background:

Synonyms: BBAP, E3 ubiquitin-protein ligase DTX3L, B-lymphoma- and BAL-associated protein, Protein deltex-3-like, Rhysin-2, Rhysin2, DTX3L

Background: Ubiquitin ligase that mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1), in response to DNA damage. Protects cells exposed to DNA-damaging agents. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me). Involved in the recruitment of 53BP1/TP53BP1 to sites of DNA damage by mediating H4K91ub1 formation. In concert with PARP9, plays a role in PARP1-dependent DNA damage repair. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.

Gene ID: 151636

UniProt: Q8TDB6

Pathways: Notch Signaling