CILP antibody (AA 1051-1184) (HRP)

Catalog No. ABIN2559701

This Rabbit Polyclonal antibody specifically detects CILP in WB, ELISA, IHC (fro) and IHC (p). It exhibits reactivity toward Human and Rat.

Quick Overview for CILP antibody (AA 1051-1184) (HRP) (ABIN2559701)

Target: CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CILP antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CILP Antibody (HRP)

Binding Specificity: AA 1051-1184

Specificity: The immunogen falls within the Cartilage intermediate layer protein 1 C2 region.

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Cow,Sheep,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CILP

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CILP

Target: CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))

Alternative Name: CILP

Background:

Synonyms: CILP-1, HsT18872, Cartilage intermediate layer protein 1, Cartilage intermediate-layer protein, CILP, UNQ602/PRO1188

Background: Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage.

Gene ID: 8483

UniProt: O75339