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CD4 antibody (PE-Cy7)

CD4 Reactivity: Mouse FACS Host: Rat Monoclonal RM4-5 PE-Cy7

Catalog No. ABIN2567677

Datasheet as PDF

Target See all CD4 Antibodies

CD4
Reactivity
340

207

119

48

24

20

19

18

17

10

10

10

10

10

10

9

9

9

8

5

4

2

1

1

1

1

1
Mouse
Host
354

147

111

3

3

1

1
Rat
Compatible Secondaries
Clonality
474

138

5
Monoclonal
Conjugate
206

68

60

42

33

12

11

9

9

9

9

9

8

8

7

5

5

5

5

5

5

5

5

5

5

5

5

4

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1
This CD4 antibody is conjugated to PE-Cy7
Application
498

190

124

100

94

69

63

42

39

31

30

29

10

8

8

7

6

6

5

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS)

Specificity

The RM4-5 monoclonal antibody specifically reacts with mouse CD4, also known as L3T4, a 55 kDa differentiation antigen expressed by the majority of thymocytes, subpopulations of mature T cells (like major histocompatibility complex class II-restricted T lymphocytes), a subset of natural killer T cells, and on pluripotent hematopoietic stem cells.

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Clone

RM4-5

Isotype

IgG2a kappa

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CD4 Reactivity: Human, Mouse, Rat, Chicken, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

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CD4 Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Pig, Cat, Cow, Rabbit, Sheep, Guinea Pig, Goat, Horse, Avian, Donkey, Hamster WB, IHC Host: Goat Polyclonal unconjugated

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CD4 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

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CD4 Reactivity: Human FACS, WB, CyTOF Host: Mouse Monoclonal MEM-241 unconjugated

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CD4 Reactivity: Mouse FACS, IP, IHC (fro), ICC, Func Host: Rat Monoclonal GK1-5 unconjugated

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CD4 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 FITC

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 PE

anti-CD4 (CD4) antibody (PE)
CD4 Reactivity: Rat FACS Host: Mouse Monoclonal OX-35 PE

anti-CD4 (CD4) (AA 26-200) antibody
CD4 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Liquid

Concentration

0.2 mg/mL

Buffer

Phosphate-buffered aqueous solution, ≤0.09 % Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Storage

4 °C

Storage Comment

The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.
Target

CD4

Alternative Name

CD4 (CD4 Products)

Background

The RM4-5 monoclonal antibody specifically reacts with mouse CD4, also known as L3T4, a 55 kDa differentiation antigen expressed by the majority of thymocytes, subpopulations of mature T cells (like major histocompatibility complex class II-restricted T lymphocytes), a subset of natural killer T cells, and on pluripotent hematopoietic stem cells. CD4 binds to the major histocompatibility complex class II (MHC class II) and enhances T lymphocyte development and mature T cells functions. In T lymphocytes, CD4 binds to the cytoplasmic tail of enzyme tyrosine kinase (p56lck).Binding of RM4-5 is blocked by the anti-mouse CD4 clone GK1.5.

Gene ID

12504

UniProt

P06332

Pathways

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events