MHC Class II antibody

Catalog No. ABIN2568358

The Rat Monoclonal anti-MHC Class II antibody (Clone M5-114-15-2) (ABIN2568358) specifically detects MHC Class II in FACS, WB, IHC, IP, Func and EM. The antibody is reactive with Mouse samples.

Quick Overview for MHC Class II antibody (ABIN2568358)

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MHC Class II antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Functional Studies (Func), Electron Microscopy (EM)

Clone: M5-114-15-2

Product Details anti-MHC Class II Antibody

Specificity: The M5/114.15.2 monoclonal antibody or (M5/114) is specific for the mouse major histocompatibility complex class II, specific for the I-A and I-E subregion-encoded glycoproteins (alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, but not the I-Af, I-Ak, nor I-As antigens).

Purification: The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered aqueous solution, ≤0.09 % Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Storage: 4 °C

Storage Comment: The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.

Target Details for MHC Class II

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Alternative Name: MHC Class II

Background: The M5/114.15.2 monoclonal antibody or (M5/114) is specific for the mouse major histocompatibility complex class II, specific for the I-A and I-E subregion-encoded glycoproteins (alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, but not the I-Af, I-Ak, nor I-As antigens). The antibody also reacts with cells from the H-2p or H-2r haplotypes mice. The polymorphic determinant represented by the mouse major histocompatibility complex class II (MHC Class II) is expressed by the immune cells bearing the specified alloantigens, like T and B lymphocytes, monocytes, dendritic cells, and macrophages. The antibody doesn't react with the NOD H-2g7 haplotype alloantigens. M5/114 mAb inhibits I-A-restricted T cell responses of the Haplotypes H-2b, H-2d, H-2q, and H-2u.

Gene ID: 14961

UniProt: P14483