PKA alpha/beta Cat (pThr197) antibody
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- Target
- PKA alpha/beta Cat
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Binding Specificity
- pThr197
- Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Specificity
- The antibody detects endogenous levels of PKA alpha/beta CAT only when phosphorylated at threonine 197.
- Purification
- Antibodies were purified by affinity-chromatography using epitope-specific peptide.
- Immunogen
- PKA alpha/beta CAT (phospho Thr197) antibody was raised against a peptide sequence around phosphorylation site of threonine 197 (R-T-W (p)-T-L) derived from Human PKA alpha/beta CAT.
- Isotype
- IgG
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- Application Notes
- Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store antibody at -20°C for up to one year.
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- Target
- PKA alpha/beta Cat
- Background
- Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.
- Molecular Weight
- 40 kDa
- Gene ID
- 5566
- NCBI Accession
- NP_002721
- UniProt
- P17612
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