FOXP3 antibody

Catalog No. ABIN2602569

The Rat Monoclonal anti-FOXP3 antibody (Clone MF333F) (ABIN2602569) specifically detects FOXP3 in WB, IHC, ELISA, IF and ICC. The antibody is reactive with Mouse samples.

Quick Overview for FOXP3 antibody (ABIN2602569)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This FOXP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: MF333F

Product Details anti-FOXP3 Antibody

Specificity: Recognizes mouse FOXP3. Detects a band of ~46-49  kDa by Western blot.

Purification: Protein G purified from tissue culture supernatant

Immunogen: Recombinant mouse FOXP3.

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA (1:2000 - 1:10000), ICC, IF, IHC, WB (1:2000 - 1:5000)

Usage: ELISA - Direct or indirect: 1:2000-1:10000. Western blot - 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05 % Tween 20 and 5 % skim milk. Suggested incubation time is 1 hour at rooom temperature.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, no preservatives.

Preservative: Without preservative

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: FOXP3

Background: Name/Gene ID: FOXP3

Synonyms: FOXP3, AIID, DIETER, Forkhead box protein P3, JM2, Forkhead box P3, FOXP3delta7, XPID, PIDX, SCURFIN

Gene ID: 50943

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation