Caprin-1 antibody (Internal Region)

Catalog No. ABIN2613398

Product Details anti-Caprin-1 Antibody

Target: Caprin-1 (Cell Cycle Associated Protein 1 (Caprin-1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Caprin-1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: CAPRIN1

Sequence: EQRPQKEPID Q

Specificity: This antibody is expected to recognize both reported isoforms (NP_005889.3, NP_976240.1).

Cross-Reactivity: Dog, Human, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5 μg/mL.

Western Blot: Approx 80+140 kDa bands observed in lysates of cell line Caco-2, HeLa, Jurkat and K562 (calculated MW of 78.4 kDa according to NP_005889.3). The ~140 kDa band corresponds to the reported p137 (Ellis and Luzio, J Biol Chem. 1995 Sep 1,270(35):2

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Caprin-1

Target: Caprin-1 (Cell Cycle Associated Protein 1 (Caprin-1))

Alternative Name: CAPRIN1 (Caprin-1 Products)

Background: CAPRIN1, cell cycle associated protein 1, GPIAP1, GPIP137, M11S1, RNG105, p137GPI, GPI-anchored membrane protein 1, GPI-anchored protein p137, GPI-p137, RNA granule protein 105, activation/proliferation-associated protein 1, caprin 1, caprin-1, cell cycle

Gene ID: 4076, 362173

NCBI Accession: NP_005889, NP_976240