CD27 antibody (AA 161-175)

Catalog No. ABIN2613401

This Goat Polyclonal antibody specifically detects CD27 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for CD27 antibody (AA 161-175) (ABIN2613401)

Target: CD27 (CD27 Molecule (CD27))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD27 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CD27 Antibody

Binding Specificity: AA 161-175

Purpose: CD27 (aa161-175)

Sequence: QTLADFRQLP ARTLS

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 45 kDa band observed in Human Spleen, Tonsil and Thymus lysates (calculated MW of 29.2 kDa according to NP_001233.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27

Background: CD27, CD27 Molecule, S152, S152. LPFS2, T14, TNFRSF7, Tp55, CD27 antigen, CD27L receptor, T cell activation antigen S152, T-cell activation antigen CD27, tumor necrosis factor receptor superfamily, member 7

Gene ID: 939

NCBI Accession: NP_001233

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints