CD74 antibody (Internal Region)

Catalog No. ABIN2613403

This anti-CD74 antibody is a Goat Polyclonal antibody detecting CD74 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for CD74 antibody (Internal Region) (ABIN2613403)

Target: CD74 (HLA-DR-gamma (CD74))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD74 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-CD74 Antibody

Binding Specificity: Internal Region

Purpose: CD74

Sequence: HSLEQKPTDA PPK

Specificity: This antibody is expected to recognize isoform a (NP_001020330.1) and isoform b (NP_004346.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 5 μg/mL.

Western Blot: Approx 33 kDa band observed in Human Tonsil lysates (calculated MW of 33.5 kDa according to NP_001020330.1). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB: Meza-Romero

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: CD74

Background: CD74, DHLAG, HLADG, Ia-GAMMA, CD74 Molecule, major histocompatibility complex, class II invariant chain, CD74 antigen, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), HLA-DR-gamma, Ia-associated invar

Gene ID: 972

NCBI Accession: NP_001020330, NP_004346

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints