VCP antibody (Internal Region)
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- Target See all VCP Antibodies
- VCP (Valosin Containing Protein (VCP))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This VCP antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purpose
- CDC48 / YDL126C
- Sequence
- HTKNMKLADD VDLE
- Specificity
- This antibody may cross react in many other species.
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Rat, Saccharomyces cerevisiae
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Isotype
- IgG
- Top Product
- Discover our top product VCP Primary Antibody
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anti-Valosin Containing Protein (VCP) (N-Term) antibody
VCP Reactivity: Human WB, IF, IP, IHC (p), ICC, IHC (wm) Host: Rabbit Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) (C-Term) antibodyKO Validated VCP Reactivity: Human WB, IF, IP, IHC (p), ICC, IHC (fro), IHC (wm) Host: Rabbit Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) (AA 125-371) antibodyVCP Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) (AA 1-588) antibodyVCP Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 4A8 unconjugated
anti-Valosin Containing Protein (VCP) (AA 647-806) antibodyVCP Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) antibodyVCP Reactivity: Human, Mouse WB, ICC, FACS Host: Mouse Monoclonal Hs-14 unconjugated
anti-Valosin Containing Protein (VCP) antibodyVCP Reactivity: Human WB, ELISA, IHC, IF, IP Host: Rabbit Monoclonal 5H12 unconjugated Recombinant Antibody
anti-Valosin Containing Protein (VCP) (AA 732-760), (C-Term) antibodyVCP Reactivity: Human, Mouse, Rat WB, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) (AA 749-766), (C-Term) antibodyVCP Reactivity: Human, Rat WB, IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) (AA 732-760) antibodyVCP Reactivity: Human, Mouse, Rat WB, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 5 μg/mL.
Western Blot: Approx 100 kDa band observed in lysates of cell line HeLa (calculated MW of 92.0 kDa according to NP_010157.1). Recommended concentration: 0.01-0.03 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:128000.
- Comment
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- VCP (Valosin Containing Protein (VCP))
- Alternative Name
- CDC48 (VCP Products)
- Background
- CDC48, CDC48p, YDL126C, VCP, transitional endoplasmic reticulum ATPase, valosin containing protein, p97, TERA
- Gene ID
- 7415, 269523, 116643
- NCBI Accession
- NP_010157
- Pathways
- ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway
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