VCP antibody (Internal Region)

Catalog No. ABIN2613406

This anti-VCP antibody is a Goat Polyclonal antibody detecting VCP in WB, IHC and ELISA. Suitable for Human.

Quick Overview for VCP antibody (Internal Region) (ABIN2613406)

Target: VCP (Valosin Containing Protein (VCP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This VCP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-VCP Antibody

Binding Specificity: Internal Region

Purpose: CDC48 / YDL126C

Sequence: HTKNMKLADD VDLE

Specificity: This antibody may cross react in many other species.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat, Saccharomyces cerevisiae

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 5 μg/mL.

Western Blot: Approx 100 kDa band observed in lysates of cell line HeLa (calculated MW of 92.0 kDa according to NP_010157.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VCP

Target: VCP (Valosin Containing Protein (VCP))

Alternative Name: CDC48

Background: CDC48, CDC48p, YDL126C, VCP, transitional endoplasmic reticulum ATPase, valosin containing protein, p97, TERA

Gene ID: 7415, 269523, 116643

NCBI Accession: NP_010157

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway