EEA1 antibody (AA 821-835)

Catalog No. ABIN2613419

This Goat Polyclonal antibody specifically detects EEA1 in WB, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for EEA1 antibody (AA 821-835) (ABIN2613419)

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This EEA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-EEA1 Antibody

Binding Specificity: AA 821-835

Purpose: EEA1 (aa821-835)

Sequence: QETKIQHEEL NNRIQ

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 200 kDa band observed in lysates of cell line HEK293 (calculated MW of 162 kDa according to NP_003557.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL. Primary incubatiion 1 hour a

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence :Strong expression of the protein seen in the vesicles/cytoplasm of NIH3T3 cells. Recommended concentration: 5μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EEA1

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Alternative Name: EEA1

Background: EEA1, early endosome antigen 1, MST105, MSTP105, ZFYVE2, early endosome antigen 1, 162kD, early endosome-associated protein, endosome-associated protein p162, zinc finger FYVE domain-containing protein 2

Gene ID: 8411

NCBI Accession: NP_003557

Pathways: Toll-Like Receptors Cascades