IL3RA antibody (Internal Region)

Catalog No. ABIN2613443

This Goat Polyclonal antibody specifically detects IL3RA in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for IL3RA antibody (Internal Region) (ABIN2613443)

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IL3RA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-IL3RA Antibody

Binding Specificity: Internal Region

Purpose: IL3RA / CD123

Sequence: RQQYECLHYK TD

Specificity: This antibody is expected to recognize both reported isoforms (NP_002174.1, NP_001254642.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 5 μg/mL.

Western Blot: Approx 43 kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 43.3 kDa according to NP_002174.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IL3RA

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Alternative Name: IL3RA

Background: IL3RA, interleukin 3 receptor, alpha (low affinity), CD123, IL3R, IL3RAY, IL3RX, IL3RY, hIL-3Ra, CD123 antigen, IL-3 receptor alpha SP2 isoform, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA, interleukin-3 receptor subunit alpha

Gene ID: 3563

NCBI Accession: NP_002174, NP_001254642

Pathways: JAK-STAT Signaling