NME1 antibody (C-Term)

Catalog No. ABIN2613461

This Goat Polyclonal antibody specifically detects NME1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for NME1 antibody (C-Term) (ABIN2613461)

Target: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NME1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-NME1 Antibody

Binding Specificity: C-Term

Purpose: NME1

Sequence: DYTSCAQNWI YE

Specificity: This antibody is expected to recognize both reported isoforms (NP_937818.1, NP_000260.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 20+18 kDa bands observed in lysates of cell lines A549, HeLa and HepG2 (calculated MW of 19.7 kDa according to NP_937818.1 and 17.1 kDa according to NP_000260.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NME1

Target: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Alternative Name: NME1

Background: NME1, AWD, GAAD, NB, NBS, NDPK-A, NDPKA, NM23, NM23-H1, non-metastatic cells 1, protein (NM23A) expressed in, NDP kinase A, OTTHUMP00000174772

Gene ID: 4830

NCBI Accession: NP_937818, NP_000260

Pathways: Apoptosis, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process