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Vimentin antibody (C-Term)

Name: Vimentin antibody (C-Term)
Brand: antibodies-online
SKU: ABIN2613493
Price: 575.06 USD
Availability: InStock

This anti-Vimentin antibody is a Goat Polyclonal antibody detecting Vimentin in WB, IHC, ELISA, IF and FACS. Suitable for Human, Mouse and Rat.

ABIN2613493 (0. (Vimentin antibody (C-Term))

(ABIN2613493) (5 μg/mL) staining of paraffin embedded Human Kidney. (Vimentin antibody (C-Term))

IHC

ABIN2613493 (2µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). (Vimentin antibody (C-Term))

ABIN2613493 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. (Vimentin antibody (C-Term))

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0. (Vimentin antibody (C-Term))

FACS

5 images

Catalog No. ABIN2613493

Quick Overview for Vimentin antibody (C-Term) (ABIN2613493)

Target

See all Vimentin (VIM) Antibodies

Vimentin (VIM)

Reactivity

Human, Mouse, Rat

Host

Goat

Clonality

Polyclonal

Conjugate

This Vimentin antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade

Verified

Discover our top product VIM Primary Antibody

Binding Specificity
34

32

16

16

12

10

9

7

5

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term

Purpose

Vimentin

Sequence

QVINETSQHH DDLE

Cross-Reactivity

Cow, Dog, Human, Mouse, Pig, Rat

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype

IgG
anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, IF Host: Mouse Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, IF, IP Host: Rabbit Monoclonal unconjugated

Application Notes

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.
Western Blot: Approx 55 kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60 kDa band in Rat Ovary lysates (calculated MW of 53.7 kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1).
Peptide ELISA: antibody detection limit dilution 1:4000.

Comment

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. Recommended conc

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Minimize freezing and thawing.

Storage

-20 °C

Storage Comment

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Target

Vimentin (VIM)

Alternative Name

VIM

Background

VIM, vimentin, FLJ36605

Gene ID

7431, 22352, 81818

NCBI Accession

NP_003371

Pathways

Caspase Cascade in Apoptosis