ATP2A2 antibody (AA 665-679)

Catalog No. ABIN2617317

The Rabbit Polyclonal anti-ATP2A2 antibody is suitable to detect ATP2A2 in samples from Human, Rat, Mouse, Rabbit, Monkey, Guinea Pig, Horse, Xenopus laevis and Bat. It has been validated for WB, IHC and IHC (p).

Quick Overview for ATP2A2 antibody (AA 665-679) (ABIN2617317)

Target: ATP2A2 (ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2))

Reactivity: Human, Rat, Mouse, Rabbit, Monkey, Guinea Pig, Horse, Xenopus laevis, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP2A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATP2A2 Antibody

Binding Specificity: AA 665-679

Specificity: Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart. .

Cross-Reactivity (Details): No cross reactivity with other proteins.

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SERCA2 ATPase(665-679aa QRDACLNARCFARVE), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2 HPO4, 0.05 mg Thimerosal, 0.05 mg sodium azide per 100 μg antibody.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid freeze-thaw cycles.

Target Details for ATP2A2

Target: ATP2A2 (ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2))

Alternative Name: ATP2A2 / SERCA2

Background: Name/Gene ID: ATP2A2
Subfamily: ATPase - P type, type IIA
Family: Transporter

Synonyms: ATP2A2, ATP2B, Calcium pump 2, DAR, SR Ca(2+)-ATPase 2, Cardiac Ca2+ ATPase, DD, SERCA2

Gene ID: 488

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Ribonucleoside Biosynthetic Process