CMA1 antibody

Catalog No. ABIN2619354

The Mouse Monoclonal anti-CMA1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect CMA1 in samples from Human.

Quick Overview for CMA1 antibody (ABIN2619354)

Target: CMA1 (Chymase 1, Mast Cell (CMA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CMA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CMA1 Antibody

Brand: IHC-plus™

Specificity: Recognizes human mast cells in routinely fixed tissues, and has been used to study the distribution of mast cells in skin, synovium, lung and heart. Binding of this antibody to chymase is not reduced in the presence of heparin, suggesting that the antibody does not bind to the heparin-binding domain of chymase.

Purification: Protein G purified

Immunogen: Purified human skin chymase. Cellular Localization: Cytoplasmic.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (1:100), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Immunohistochemistry (Formalin/paraffin): Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Tonsil.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CMA1

Target: CMA1 (Chymase 1, Mast Cell (CMA1))

Alternative Name: CMA1 / MCT1 / Chymase

Background: Name/Gene ID: CMA1
Subfamily: Serine S1
Family: Protease

Synonyms: CMA1, Chymase, Chymase, heart, Chymase, mast cell, CYH, Mast cell chymase, Mcpt5, MCT1, Mast cell protease 5, Mast cell protease I, Heart chymase, Alpha-chymase, Chymase 1, Chymase 1, mast cell, CYM

Gene ID: 1215

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Carbohydrate Homeostasis