Glypican 3 antibody (AA 324-373)

Catalog No. ABIN2621458

This anti-Glypican 3 antibody is a Rabbit Polyclonal antibody detecting Glypican 3 in WB, IHC and IHC (p). Suitable for Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit, Bat, Monkey and Pig.

Quick Overview for Glypican 3 antibody (AA 324-373) (ABIN2621458)

Target: Glypican 3 (GPC3)

Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit, Bat, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glypican 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Glypican 3 Antibody

Binding Specificity: AA 324-373

Brand: IHC-plus™

Specificity: Human GPC3 / Glypican 3

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa324-373 of human GPC3 (P51654, NP_004475). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Platypus (92%), Turkey, Zebra finch, Chicken (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (2.5 μg/mL)

Usage: Recommended conditions for Immunohistochemistry: For FFPE tissues, perform steam-based antigen retrieval using Tris/EDTA buffer at  pH 9.0.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Glypican 3

Target: Glypican 3 (GPC3)

Alternative Name: GPC3 / Glypican 3

Background: Name/Gene ID: GPC3

Synonyms: GPC3, DGSX, Glypican proteoglycan 3, Glypican 3, Glypican-3, GTR2-2, Intestinal protein OCI-5, SGB, SGBS1, SGBS, MXR7, OCI-5, OCI5, SDYS, Secreted glypican-3, Heparan sulphate proteoglycan

Gene ID: 2719

NCBI Accession: NP_004475

Pathways: Glycosaminoglycan Metabolic Process