CTNNB1 antibody (AA 108-121)

Catalog No. ABIN2630016

The Goat Polyclonal anti-CTNNB1 antibody has been validated for WB, IHC, ELISA and IF. It is suitable to detect CTNNB1 in samples from Human.

Quick Overview for CTNNB1 antibody (AA 108-121) (ABIN2630016)

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CTNNB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-CTNNB1 Antibody

Binding Specificity: AA 108-121

Purpose: CTNNB1 / catenin beta-1 (aa108-121)

Sequence: QIPSTQFDAA HPTN

Specificity: Reported variants represent identical protein: NP_001091679.1, NP_001895.1, NP_001091680.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Adrenal Gland. Recommended concentration: 5 μg/mL.

Western Blot: Approx 100 kDa band observed in lysates of cell line HeLa (calculated MW of 85.5 kDa according to NP_001895.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature..

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Expression of the protein seen in the cytoplasm/Plasma Membrane of U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Alternative Name: CTNNB1

Background: CTNNB1, catenin (cadherin-associated protein), beta 1, 88 kDa, CTNNB, MRD19, armadillo, catenin beta-1

Gene ID: 1499, 12387, 84353

NCBI Accession: NP_001895

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2