toxA antibody

Catalog No. ABIN263570

The Mouse Monoclonal anti-toxA antibody is suitable to detect toxA in samples from Clostridium. It has been validated for EIA.

Quick Overview for toxA antibody (ABIN263570)

Target: toxA (Clostridium Difficile Toxin A (toxA))

Reactivity: Clostridium

Host: Mouse

Clonality: Monoclonal

Conjugate: This toxA antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Clone: PCG4-1

Product Details anti-toxA Antibody

Characteristics: Synonyms: toxA, tcdA

Purification: Protein A Chromatography.

Immunogen: C. difficile Toxin A. Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Isotype: IgG2a

Application Details

Application Notes: ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.16 mg/mL (OD280 nm, E1% = 14)

Buffer: 10 mM Sodium Phosphate, pH 7.4 ± 0.2 containing 150 mM Sodium Chloride, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -80 °C

Storage Comment: Store the antibody at -40 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for toxA

Target: toxA (Clostridium Difficile Toxin A (toxA))

Alternative Name: Clostridium Difficile Toxin A

Background: Clostridium difficile, or C. difficile, a gram positive anaerobic bacterium, is the major causative agent of colitis and diarrhoea that can occur following antibiotic intake. C. difficile infection is one of the most common hospital acquired infections. When C. difficile colonize the gut, they release two potent toxins, toxin A and toxin B, which bind to certain receptors in the lining of the colon and ultimately cause diarrhoea and inflammation of the large intestine.Synonyms: tcdA, toxA

UniProt: P16154