APOB antibody

Catalog No. ABIN263702

The Mouse Monoclonal anti-APOB antibody has been validated for RIA and EIA. It is suitable to detect APOB in samples from Human.

Quick Overview for APOB antibody (ABIN263702)

Target: APOB (Apolipoprotein B (APOB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APOB antibody is un-conjugated

Application: Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Clone: 057-10031

Product Details anti-APOB Antibody

Specificity: This antibody is specific for Apolipoprotein B (Apo B). Does not cross-react with Apo A-I or Apo A-II in competitive RIA.

Characteristics: Synonyms: ApoB, Apo-B, ApoB100, ApoB-100

Purification: Protein A chromatography,

Purity: > 90 % pure by SDS-PAGE

Sterility: 0.2 μm filtered

Immunogen: Human low density lipoprotein

Isotype: IgG2b

Application Details

Application Notes: RIA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 5.0 mg/mL (OD280nm, E0.1% = 1.4)

Buffer: 10 mM Phosphate, pH 7.4 containing 150 mM Sodium Chloride, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 to -80 °C for longer. Ifaliquoted for long term storage, fill volume should be equal to or greater than 50% of thenominal fill volume of the vial used. Avoid repeated freeze/thaw cycles.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for APOB

Target: APOB (Apolipoprotein B (APOB))

Alternative Name: Apolipoprotein B (Apo B)

Background: Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 516 kDa and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800 mg/L.Synonyms: Apo-B, ApoB, ApoB-100, ApoB100

Gene ID: 338

UniProt: P04114

Pathways: Lipid Metabolism