IL-8 antibody

Catalog No. ABIN263860

This Mouse Monoclonal antibody specifically detects IL-8 in WB, EIA and Func. It exhibits reactivity toward Human.

Quick Overview for IL-8 antibody (ABIN263860)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-8 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Clone: BDI670

Product Details anti-IL-8 Antibody

Characteristics: Synonyms: CXCL8, Protein 3-10C, Emoctakin, GCP1, MDNCF, MONAP, NAP1, Monocyte-derivedneutrophil chemotactic factor, T-cell chemotactic factor, Neutrophil-activating protein 1,Granulocyte chemotactic protein 1, Monocyte-derived neutrophil-activating peptide

Purification: Protein G Chromatography.

Immunogen: Purified recombinant human IL-8

Isotype: IgG1

Application Details

Application Notes: ELISA. Neutralizing: The antibody inhibited 98.4 % of the chemotactic activity of IL-8 on RB/293cell using 5g/mL of this antibody and 10 ng/mL of human IL-8. This antibody had noinhibitory effect on MIP 1-beta and RANTES in neutrophil chemotaxis assay. Western blot: Concentration of 0.02-0.1g/mL will allow visualization of 100 ng/lane ofhuman IL-8.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL double distilled water.

Concentration: 1.0 mg/mL (prior to lyophilization)

Buffer: 0.01 M PBS, pH 7.0 without preservatives.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: Interleukin-8 / IL8

Background: Interleukin 8, IL8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL8 attracts neutrophils, basophils, and T-cells, but not monocytes. Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology.Synonyms: CXCL8, Emoctakin, GCP1, Granulocyte chemotactic protein 1, MDNCF, MONAP, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAP1, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor

UniProt: P10145

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy