Nitrotyrosine antibody

Catalog No. ABIN263971

This Mouse Monoclonal antibody specifically detects Nitrotyrosine in WB, IP, IHC (fro) and EIA. It exhibits reactivity toward .

Quick Overview for Nitrotyrosine antibody (ABIN263971)

Target: Nitrotyrosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nitrotyrosine antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 39B6

Product Details anti-Nitrotyrosine Antibody

Characteristics: Synonyms: NO-Tyrosine, Nitro-Tyrosine

Purification: Affinity Chromatography on Protein G.

Immunogen: 3-(4-Hydroxy-3-Nitrophenyl Acetamido) Propionic Acid-BSA conjugate

Isotype: IgG2a

Application Details

Application Notes: ELISA (1,6). Immunoprecipitation. Western blot (1): 0.7 μg/mL was sufficient for detection of 5 μg SN-1 treated BSA using Goatanti-Mouse IgG-HRP as the secondary antibody. Immunohistochemistry on Frozen Sections (1,5).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative in 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Nitrotyrosine

Target: Nitrotyrosine

Target Type: Chemical

Background: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an mportant component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, sinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high orrelation to disease states such as atherosclerosis, Alzheimer' disease, Parkinson's disease and amyotrophic lateral sclerosis (4).Synonyms: NO-Tyrosine, Nitro-Tyrosine