NAPSA antibody (N-Term)

Catalog No. ABIN263986

This Mouse Monoclonal antibody specifically detects NAPSA in IHC (p). It exhibits reactivity toward Human.

Quick Overview for NAPSA antibody (N-Term) (ABIN263986)

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NAPSA antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: KCG1-1

Product Details anti-NAPSA Antibody

Binding Specificity: N-Term

Specificity: Napsin A and TTF-1 reactivities were determined immunohistochemically on formalin-fixed paraffin embedded cell blocks from 50 pleural effusion (5 mesotheliomas, 10 mesothelial proliferations, 12 pulmonary, and 23 nonpulmonary metastases). TTF-1 and Napsin A were positive in 8/12 and 10/12 pulmonary adenocarcinomas, respectively. The specificity of this antibody for primary lung adenocarcinoma is 47 out of 58 (81 %), primary lesions were positive. This antibody may be a useful tool as a tumor marker for primary lung adenocarcinoma.

Characteristics: Synonyms: NAPSA, NAP1, Napsin-1, Aspartyl protease 4, TA01/TA02

Purification: Purified

Immunogen: Synthetic peptide from N terminus of Human Napsin A

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: Use at 1/50-1/100 dilution in an ABC method(30 minutes at room temperature). However, depending upon the fixation conditions andthe staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Lung Adenocarcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for NAPSA

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Alternative Name: Napsin-A

Background: Napsin is found in two isoforms, A and B, with highly homologous gene sequences. Napsin A has a molecular weight of 35.0 kDa and is also known as TA02. Napsin A is an aspartic proteinase which is expressed in the lung and involved in processing surfactant protein B (SP-B). It is also expressed in the kidney.Synonyms: Aspartyl protease 4, NAP1, NAPSA, Napsin-1, TA01/TA02

Gene ID: 9476

UniProt: O96009

Pathways: Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis