alpha-Thrombin antibody

Catalog No. ABIN264120

Product Details anti-alpha-Thrombin Antibody

Target: alpha-Thrombin

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha-Thrombin antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Purification: Ammonium sulfate precipitation, ion-exchange chromatography and gel filtration

Purity: > 95 % pure (SDS-PAGE)

Immunogen: Purified human thrombin

Clone: BDI905

Isotype: IgG1

Application Details

Application Notes: ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 4.4mg/mL (OD280nm, E1% = 14)

Buffer: 50 % Glycerol/water (vol/vol)

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for alpha-Thrombin

Target: alpha-Thrombin

Alternative Name: Thrombin (alpha-Thrombin Products)

Synonyms: PT antibody, RPRGL2 antibody, THPH1 antibody, coagulation factor II, thrombin antibody, F2 antibody

Background: Thrombin is an active enzyme in the earliest steps of the blood clot formation, generated from its circulating inactive precursor prothrombin. Thrombin is a glycoprotein formed by two peptides chains of 36 and 259 amino acids linked by disulfure bonds. Three important sites have been identified on the surface of the enzyme: The catalytic site that confers to the molecule its serine protease activity, the exosite one responsible for the binding of the substrate (fibrinogen or thrombin receptor) and the exosite two responsible for the binding of antithrombin III and inactivation of thrombin. The molecular mass of thrombin is 37 kDa.