TNNI3 antibody (AA 65-74)

Catalog No. ABIN264150

The Mouse Monoclonal anti-TNNI3 antibody (Clone B158M) (ABIN264150) specifically detects TNNI3 in WB, IP, EIA and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for TNNI3 antibody (AA 65-74) (ABIN264150)

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNNI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B158M

Product Details anti-TNNI3 Antibody

Binding Specificity: AA 65-74

Specificity: This antibody reacts with free cardiac troponin I (cTnI). Does not react with cTnI forming complexes with other troponin components. Not affected by phosphorylation. Recognizes an epitope between amino acid residues 65 and 74 of cTnI. No crossreactivity with skeletal muscle cTnI.

Characteristics: Synonyms: TNNI3, TNNC1

Purification: Protein A chromatography

Immunogen: Free human cTnI and native human cTn complex.

Isotype: IgG1

Application Details

Application Notes: ImmunoassayImmunoprecipitation. Immunohistochemistry. Immunoaffinity purification. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.4 containing 0,09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for TNNI3

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Alternative Name: Cardiac Troponin I

Background: Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.Synonyms: TNNC1, TNNI3

Gene ID: 7137

UniProt: P19429