CD2 antibody (APC)

Catalog No. ABIN264177

This anti-CD2 antibody is a Mouse Monoclonal antibody detecting CD2 in FACS. Suitable for Human.

Quick Overview for CD2 antibody (APC) (ABIN264177)

Target: CD2

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD2 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: T6-3

Product Details anti-CD2 Antibody (APC)

Characteristics: Synonyms: T11/Leu-5, LFA-2, LFA-3 receptor, Erythrocyte receptor, Rosette receptor, SRBC, T-cellsurface antigen CD2, T-cell surface antigen T11/Leu-5

Immunogen: Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/cmice immunized with T Lymphocytes activated by mixed lymphocyte culture.

Isotype: IgG2a

Application Details

Application Notes: Suitable for Monitoring of T cells in peripheral blood, Analysis of NK subsets, Study of Tcell activation. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: PBMC: Add 10 ul of Anitbody/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of Antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at roomtemperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturer's instructions for Lysed Whole Blood andImmunofluorescence analysis with a Flow cytometer or microscope.

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.08 % sodium azide as preservative and 0.2 % protein carrier.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for CD2

Target: CD2

Alternative Name: CD2

Background: Identification of human T cells and subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen.Synonyms: Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, SRBC, T-cell surface antigen CD2, T-cell surface antigen T11/Leu-5

UniProt: P06729