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CD33 antibody (PE)

CD33 Reactivity: Human FACS Host: Mouse Monoclonal FOS PE

Catalog No. ABIN264253

Datasheet as PDF

Target See all CD33 Antibodies

CD33
Reactivity
159

35

15

13

7

6

3

1
Human
Host
92

73
Mouse
Compatible Secondaries
Clonality
89

75

1
Monoclonal
Conjugate
54

15

13

12

9

5

5

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD33 antibody is conjugated to PE
Application
104

98

39

30

26

26

22

22

16

13

9

8

6

4

4

2

2

2

1

1

1

1
Flow Cytometry (FACS)

Specificity

This antibody recognises human Myeloid.

Characteristics

Synonyms: Siglec-3, gp67, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3

Clone

FOS

Isotype

IgG1

Top Product

Discover our top product CD33 Primary Antibody
anti-CD33 (CD33) (AA 101-200) antibody
CD33 Reactivity: Human, Mouse FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD33 (CD33) (AA 15-237) antibody
CD33 Reactivity: Human FACS, WB, ELISA, IHC Host: Mouse Monoclonal 2C6B7 unconjugated

anti-CD33 (CD33) (AA 18-240) antibody
CD33 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD33 (CD33) (AA 49-259) antibody
CD33 Reactivity: Human WB, IF, IHC Host: Rabbit Polyclonal unconjugated

anti-CD33 (CD33) (AA 261-364) antibody
CD33 Reactivity: Human FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD33 (CD33) (AA 1-364) antibody
CD33 Reactivity: Human WB, ELISA, RNAi Host: Mouse Monoclonal 2D12-G4 unconjugated

anti-CD33 (CD33) (AA 18-269) antibody
CD33 Reactivity: Human FACS, ELISA Host: Rabbit Monoclonal DM77 unconjugated

anti-CD33 (CD33) antibody
CD33 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC, CyTOF Host: Mouse Monoclonal WM53 unconjugated

anti-CD33 (CD33) antibody
CD33 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC, Func, CyTOF Host: Mouse Monoclonal WM53 unconjugated

anti-CD33 (CD33) antibody (Biotin)
CD33 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal HIM3-4 Biotin

Application Notes

Flow Cytometry. Characterization of subtypes of leukemias and lymphomas. Studies of AIDS virus infection. Myeloid cell function studies. Analysis of hematopoietic maturation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol

PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze.

Restrictions

For Research Use only
Buffer

PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Expiry Date

12 months
Target

CD33

Alternative Name

CD33 / SIGLEC3 (CD33 Products)

Synonyms

SIGLEC-3 antibody, SIGLEC3 antibody, p67 antibody, Siglec-3 antibody, gp67 antibody, CD33 molecule antibody, CD33 antigen antibody, CD33 antibody, Cd33 antibody

Background

CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67 kDa M.W. surface antigen. CD33 is also found on CFU-mix, CFU-GM, CFU-Meg, a portion of BFU-E, myeloblasts, promyelocytes, myelocytes, metamyelocytes but not early precursors.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67

Gene ID

945

UniProt

P20138